Table 1. Amount and Subcellular Distribution of Compounds in Arabidopsis.
Compounds | Amount (nmol g FW−1) | Plastid (%) | Cytosol (%) | Vacuole (%) |
---|---|---|---|---|
3PGAa | 200 ± 45 | 44 | 56 | 0 |
2PGAa | 20 ± 4.5 | 0 | 100 | 0 |
ADPGa | 0.55 ± 0.05 | 100 | 0 | 0 |
DHAPa | 2.7 ± 0.6 | 23 | 77 | 0 |
F6Pa | 86.4 ± 14.6 | 34 | 66 | 0 |
FBPa | 8.9 ± 2.3 | 70 | 30 | 0 |
G1Pa | 11.7 ± 2.4 | 8 | 92 | 0 |
G6Pa | 173 ± 51 | 17 | 83 | 0 |
RuBPa | 46.7 ± 8.2 | 83 | 17 | 0 |
S7Pa | 28.0 ± 5.4 | 82 | 18 | 0 |
SBPa | 9.6 ± 3.0 | 75 | 25 | 0 |
UDPGa | 35.7 ± 5.7 | 0 | 100 | 0 |
R5Pa | 1.2 ± 0.2 | 49 | 51 | 0 |
X5P+Ru5Pa | 8.7 ± 2.6 | 100 | 0 | 0 |
Suc6Pa | 0.82 ± 0.36 | 0 | 100 | 0 |
PEPa | 52.5 ± 9.9 | 6 | 94 | 0 |
Gly | 543 ± 59 | 28 | 33 | 39 |
Glycerate | 169 ± 65 | 31 | 47 | 22 |
Ser | 4,265 ± 326 | 36 | 22 | 42 |
myo-inositol | 922 ± 108 | 66 | 0 | 34 |
Suc | 3,432 ± 167 | 19 | 31 | 50 |
Trehalose | 20.8 ± 3.0 | 50 | 0 | 50 |
Glu | 3,682 ± 1,042 | 30 | 42 | 28 |
Malate | 1,820 ± 547 | 0 | 0 | 100 |
2-Oxoglutarate | 63.1 ± 18.8 | 6 | 35 | 59 |
Pyruvate | 99.2 ± 35.6 | 24 | 31 | 45 |
Val | 139 ± 16 | 32 | 0 | 68 |
Ile | 38.9 ± 4.5 | 33 | 0 | 67 |
Pro | 641 ± 90 | 31 | 24 | 45 |
Thr | 459 ± 58 | 42 | 0 | 58 |
Fumarate | 1,154 ± 47 | 0 | 0 | 100 |
Asp | 1,050 ± 346 | 19 | 46 | 35 |
Phe | 53.1 ± 6.9 | 48 | 0 | 52 |
Asn | 380 ± 25 | 30 | 17 | 53 |
Fru | 1,458 ± 30 | 0 | 12 | 88 |
Glc | 2,669 ± 475 | 12 | 23 | 65 |
Arg | 164 ± 18 | 42 | 0 | 58 |
Tyr | 9.8 ± 1.2 | 45 | 0 | 55 |
Citrate | 1,916 ± 505 | 0 | 0 | 100 |
Shikimate | 35.9 ± 7.6 | 37 | 22 | 41 |
Succinate | 84.0 ± 48.2 | 4 | 17 | 79 |
Ala | 228 ± 24 | 16 | 14 | 70 |
Gln | 2,349 ± 177 | 11 | 0 | 89 |
Nitrate | 224,000 ± 4,100 | 0 | 0 | 100 |
Aconitate | 14.5 ± 5.5 | 0 | 0 | 100 |
Isocitrate | 33.5 ± 3.6 | 0 | 0 | 100 |
Lys | n.d. | n.d. | n.d. | n.d. |
Maltose | n.d. | n.d. | n.d. | n.d. |
Met | 9.9 ± 1.4 | 42 | 13 | 45 |
Data are from three independent nonaqueous gradients. 2PGA is assumed to be only in the cytosol and its amount to be 10% of the 3PGA amount. Subcellular distributions (%) were calculated using a three-compartment model (plastid, cytosol, and vacuole). For metabolites marked with an “a,” a two-compartment model was used (plastid and cytosol). n.d., not determined.