Figure 1.

dry2/sud1-1 and dry2/sud1-2 Suppress the Shoot Defects of dry2.

(A) Shoot phenotype of 3-week-old wild-type (WT), dry2, dry2/sud1-1, and dry2/sud1-2 plants grown under standard long-day conditions. Bar = 0.5 cm.

(B) Accumulation of O₂⁻ (top panel) and hydrogen peroxide (bottom panel) in 3-week-old leaves of the wild type, dry2, dry2/sud1-1, and dry2/sud1-2. Plants were stained with nitroblue tetrazolium (top panel) and DAB (bottom panel). Compared with the wild type, dry2/sud1-1, and dry2/sud1-2, dry2 accumulates very low levels of O₂⁻ and hydrogen peroxide. Bars = 0.5 cm.

(C) Stomatal conductance of the wild type, dry2, dry2/sud1-1, and dry2/sud1-2. The measurements were made 4 h after spraying the indicated ABA concentrations. The suppressors show wild-type stomatal responses to exogenous ABA compared with dry2.

(D) Pro content in wild-type, dry2, dry2/sud1-1, and dry2/sud1-2 plants grown at 35 and 90% relative humidity. FW, fresh weight.

(C) and (D) Mean ± sd, n = 9; values with the same letter are not significantly different at P < 0.05. The experiments were repeated at least three times with similar results.