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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Feb 27;190(7):3054–3058. doi: 10.4049/jimmunol.1203275

Figure 2.

Figure 2

Leptin increases RORγt transcription and IL-17 production in CD4+ T cells. Sorted RORγt-/- CD4+ T cells were stimulated with anti-CD3/CD28 Ab for 24 hours, transduced with retrovirus encoding RORγt, and restimulated with anti-CD3/CD28 Ab and scalar doses of leptin for 18 hours. The figure shows the flow cytometry results of RORγt (GFP) (a) and IL-17 expression (b) in the presence of scalar doses of leptin. c. Flow cytometry for RORγt expression in ob/ob CD4+ T cells cultured under polarizing (closed circles) or non-polarizing (open circles) conditions in the presence of scalar doses of leptin during the last 18 hours, stimulated (straight lines) or not (dashed lines) with PMA/ionomycin *P<0.05 in the comparison between polarizing and non-polarizing conditions.