Fig. 5. BSEP expression correlated with FXR isoform expression in Huh 7 and HepG2 cells.
(A) Expression levels of BSEP mRNA and (B) total FXR mRNA were quantified by TaqMan real-time PCR with GAPDH levels as internal standards. No BSEP mRNA expression was detected in HepG2. (C) Expression of FXRα1 and FXRα2 in Huh 7 and HepG2 cells. No FXRα2 expression was detected in HepG2 cells. (D) Exogenously transfected FXRα2 restored endogenous BSEP expression in a dose dependent manner in HepG2 cells.