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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Mar 6;190(7):3447–3457. doi: 10.4049/jimmunol.1200604

Figure 3. Accumulation of exudate macrophages and Ly-6Chigh monocytes in mice with targeted type II AEC injury is CCR2-dependent.

Figure 3

Diphtheria toxin was administered for 14 days per protocol to: 1) WT mice (DTR−:CCR2+/+), 2) DTR+ mice (DTR+:CCR2+/+), and 3) DTR+:CCR2−/− mice. Lung leukocytes from individual mice were isolated and antibody stained. Multi-parameter flow cytometric analysis was used to identify and enumerate total numbers of (A) ExM and (B) Ly-6Chigh monocytes. Data are mean ± SEM of 4–5 DT-treated mice per strain assayed individually. * P values <0.05 between groups at the same time point were considered significant. (C) Photomicrograph of a representative lung section (H & E staining; 40x objective) from a DT-treated DTR+ mouse deficient in CCR2. Note the relative absence of inflammation.