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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2013 Feb 20;190(7):3109–3120. doi: 10.4049/jimmunol.1203422

Figure 4.

Figure 4

The contribution of Idd3 and Idd5 sub-regions to the restoration of CD8+ T cell tolerance to endogenous islet antigen IGRP. Results were compiled from 13 independent experiments, each of which analyzed 6–12 female mice per strain, at 10–14 weeks of age. Female NOD, Idd5, Idd5.1, Idd5.2, Idd5.3, Idd5.2+5.3, Idd3, Idd3+5.1, Idd3+5.2, Idd3+5.3, Idd3+5.2+5.3, and Idd3/5 mice were infected with Vac-KdIGRP. One week later, spleens were harvested, and analyzed by FACS for CD8+ IGRP-tetramer+ cells. 30 out of 91 NOD females, 18 out of 46 Idd5 females, 29 out of 58 Idd3 females, and 30 out of 71 Idd3/5 females shown on the graph were published previously (30). These mice were included in the analysis because they were contemporaneous controls of other congenic strains that were examined at the same time. Horizontal lines depict the means. Pairs of strains were compared using unpaired t-test with Welch’s correction. NS, not significant, p-value ≥0.05. (A) Idd5.2 alone is sufficient to partially restore CD8+ T cell tolerance to self-antigen IGRP. (B) More than one Idd5 sub-regions need to be combined with Idd3 to further restore CD8+ T cell tolerance to self-antigen IGRP.