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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2013 Feb 18;190(7):3493–3499. doi: 10.4049/jimmunol.1203072

Figure 2. Localization of C5 mRNA.

Figure 2

Whole mount in situ hybridization demonstrating the localization of C5 in murine embryos at 8.5 (A–B), 9.5 (D–E), and 10.5 (G–H) days post coitum (dpc). At 8.5 dpc (A–B), C5 is localization predominantly to the neural folds (arrows, B). At 9.5 dpc and 10.5 dpc, expression is evident throughout the prosencephalon (p), mesencephalon (m), rhombencephalon (r) and within the otic vesicles (o) (D–E, G–H). Sense control embryos are also shown (C, F, I). RT-PCR confirms C5 mRNA expression throughout the period of neurulation (7.5, 8.5, 9.5 and 10.5 dpc; J). Liver was used as a positive control. ActB was used as a house-keeping gene for internal calibration. Representative examples shown (n > 3 per experiment).