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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Methods. 2012 Dec 22;59(3):261–269. doi: 10.1016/j.ymeth.2012.12.002

Figure 2. Studying protein-protein interactions by two color PALM and bivariate PCF statistics.

Figure 2

(A) Two color-PALM image of a Jurkat E6.1 cell on a TCR stimulating coverslip. The cell expressed LAT-Dronpa and PLC-γ-PAmCherry. (B) Bivariate pair-correlation function (PCF) of the identified molecules within a study region of the cell. (C) Two color-PALM image of a Jurkat E6.1 cell on a TCR stimulating coverslip. The cell expressed LATDronpa and ZAP-70-PAmCherry. (D) Bivariate PCF of the identified molecules within a study region of the cell. (E) Two color-PALM image of a Jurkat E6.1 cell on a TCR stimulating coverslip. The cell expressed LAT-Dronpa and TAC-PAmCherry. (F) Bivariate PCF of the identified molecules within a study region of the cell. (G) The extent of mixing between LAT and TAC, ZAP-70 and PLC-γ1 in multiple cells (n=9). Bars - 2 μm for whole cell images and 200nm for zoomed images.