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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Mar 4;190(7):3600–3612. doi: 10.4049/jimmunol.1201933

Figure 8. L13a controls translation of CCL22, CXCL13 and CCR3 mRNA in animal model.

Figure 8

a, Increased polyribosomal abundance of GAIT target mRNAs such as CCL22, CXCL13 and CCR3 in the macrophage of KO mice. Peritoneal macrophages harvested from 24 hr post LPS (15 mg/kg) injected control and KO mice were subjected to polyribosome fractionation by sucrose gradient. Fractions were subjected to RT-PCR analysis using specific primers (Supplemental Table S1). Increased association of the CCL22, CXCL13 and CCR3 but not actin mRNAs with the heavy polyribosome fractions were observed in KO mice compared to control mice. b, Quantifications of the results from a. Ratio of the abundance of the target mRNA and actin in polysomal and non-polysomal fractions were determined by measuring the intensities of the corresponding bands.