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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Mol Microbiol. 2013 Feb 26;88(1):20–34. doi: 10.1111/mmi.12159

Fig. 6.

Fig. 6

Gluconate substitution and a Cl requirement. (A) Matched parasite growth over 5 days in NaCl:KGluc in comparison to standard RPMI medium, each supplemented with 10% serum. Bars represent mean ± S.E.M. SYBR Green I fluorescence in arbitrary units. (B) 72 h parasite growth as a function of external [Cl] in 4suc:6KCl with 10% dialyzed serum. Reduced external Cl concentrations were achieved through isomolar substitution of KCl with K-gluconate. Mean ± S.E.M. of SYBR Green I fluorescence normalized to 100% for 4suc:6KCl (n = 6 trials).