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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Feb 18;190(7):3319–3327. doi: 10.4049/jimmunol.1203321

Figure 4. Failure of DC to up-regulate pancreatic enzymes following immunization with MUC1p as a self-antigen is recapitulated in the OVA model of self-tolerance.

Figure 4

(A) MUC1.Tg mice were immunized i.v. with PBS (ctrl), soluble MUC1p or ovalbumin (OVA) admixed with Poly-ICLC. Spleens were harvested at 24h post immunization and pooled for qRT-PCR analysis. Bars represent mean ± SEM normalized to PBS control. Data are representative of three independent experiments. (B) MUC1.Tg mice were immunized i.v. with unloaded DC (ctrl) or DC loaded with OVA (OVA). 24h post-immunization splenic DC were MACS purified for qRT-PCR analysis. Bars represent mean ± SEM normalized to ctrl. Data are representative of three independent experiments. (C) RIP.OVA mice were immunized and processed as in (B). Bars represent mean ± SEM normalized to ctrl vaccination. Data are representative of two independent experiments.