. Author manuscript; available in PMC: 2014 Apr 1.

Published in final edited form as: Lipids. 2013 Feb 12;48(4):411–419. doi: 10.1007/s11745-013-3767-5

Table 2.

Endogenous PG levels quantified in ischemic brain tissue using different extraction protocols

	Single-step methanol		Acetone/chloroform		Bligh and Dyer
	Mean ± SD ng/gww	RSD %	Mean ± SD ng/gww	RSD %	Mean ± SD ng/gww	RSD %
PGE₂	5.07 ± 0.33	6.6	4.77 ± 0.76	15.9	5.27 ± 1.36	25.8
PGD₂	1.02 ± 0.06	5.9	1.10 ± 0.19	17.2	0.90 ± 0.18	19.5

Mouse brains were analyzed 5 min after decapitation to model global brain ischemia as described in the Method section. Brain tissue was fixed before analysis by protein heat denaturing for 5 min in a boiling water bath and extracted using different extraction protocols as described in the Method section. One ng of PGE₂d₄ was used as internal standard for PG quantification by UPLC-MS/MS as described in the Method section. n=4