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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Mar 1;190(7):3410–3416. doi: 10.4049/jimmunol.1203151

Figure 3. Precursor frequency does not contribute significantly to the immunodominance of SIINFEKL-specific CD8 T-cell responses in chronic infection.

Figure 3

(A) OVA Tg mice were infected i.p. with the indicated viruses. Virus-specific CD8 T-cells were measured in the blood on day 7 and at week 18 post infection using intracellular cytokine staining. (B) C57BL/6 mice were injected with 10μg SIINFEKL peptide i.v. on days -3,-2 and -1 prior to infection with MCMV-GFP-SL8. Responses were measured in the blood on day 7 and at week 18 post infection (C) CD45.2+, CD45.1+ naive recipients received 2–5×107 unfractionated splenocytes from mice infected for 7 days with WT MCMV. Recipients were infected with WT MCMV or with MCMV-GFP-SL8 and virus -specific responses were measured in the blood at week 18 post infection. Total CD8 T-cell responses are shown on the left and percentages of CD45.2-negative donor cells contributing to either IE3 or M38 responses are shown on the right. Bars represent 4–5 mice per group. Experiments were done twice.