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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Methods. 2012 Dec 3;59(3):287–300. doi: 10.1016/j.ymeth.2012.11.006

Table 3.2.

Sedimentation properties of empty-nanodiscs.

Empty-nanodisc SE
M (kDa)a M (1 − ν̄ρ) (kDa)b Calculated ν̄ (cm3/g)c Experimental ν̄ (cm3/g)
POPC 133.194 13.9 ± 0.4 0.8899 0.886 ± 0.003
POPC/POPG 133.794 --d 0.8904 0.883d
POPG 134.394 15.1 ± 0.5 0.8908 0.879 ± 0.004
a

Expected molar mass for H7-MSP1D1 reconstituted empty-nanodiscs containing 110 lipid molecules.

b

Experimental buoyant molar mass for the monomeric empty-nanodisc. SE data collected at 10, 15 and 20 krpm for a single loading concentration were analyzed globally, using soft mass conservation, in terms of a single ideal species. The analysis and error determination were carried out without the implementation of TI noise.

c

Calculated assuming that all lipids have a partial specific volume of 0.987 cm3g−1 based on published data for POPC as described in the text [59, 89–91].

d

No data were collected for the POPC/POPG nanodiscs. The experimental partial specific volume was calculated based on data presented for the POPC only and POPG only containing empty-nanodiscs.