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CaMKII autophosphorylation in the mushroom body is not required for initial memory formation. Initial memory or learning was measured immediately (2 min) after one cycle training. Flies expressing CaMKII transgenes either throughout the mushroom body (A), in the α′/β′ neurons (B), the α/β, and γ neurons (C) or CaMKII neurons (D) all learned similar to heterozygous wildtype negative control. Data were analyzed using One-Way ANOVA followed by a Tukey's post-hoc test.
