Figure 8.
CASK and CaMKII are required for anesthesia resistant memory. (A) Flies null for CASK-β or expressing CASK-RNAi, CaMKII-T287D, or CaMKII-T287A throughout the mushroom body completely removed anesthesia resistant memory (ARM) tested 24 h after five cycles of massed training. (B) α′/β′ CASK knockdown or overexpression of CaMKII-T287D, CaMKII-T287A, or CaMKII-T306A T307A reduced ARM. (C) Mushroom body α/β and γ neuron CASK knockdown or overexpression of CaMKII-T287D, CaMKII-T287A, or CaMKII-T306A T307A did not affect ARM. Data were analyzed using One-Way ANOVA followed by a Tukey's post-hoc test. n.s. is not significant, (p > 0.05), *p < 0.05, **p < 0.01, and ***p < 0.001.