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. 2013 Mar 27;4:64. doi: 10.3389/fmicb.2013.00064

Table 1.

Respective effects of habitat, bedrock, and elevation on microbial beta-diversity, as assessed by variation partitioning on Bray–Curtis dissimilarity matrices obtained from CE-SSCP profiles.

Taxa Factors Df SS MS FR R2 Pr(>F)
Bacteria Habitat 1 0.046 0.046 5.902 0.028 0.001
Bedrock 1 0.142 0.142 18.262 0.088 0.001
Elevation 1 0.026 0.026 3.302 0.016 0.004
Habitat: bedrock 1 0.068 0.068 8.747 0.042 0.001
Habitat: elevation 1 0.005 0.005 0.697 0.003 0.666
Bedrock: elevation 1 0.015 0.015 1.876 0.009 0.070
Habitat: bedrock: elevation 1 0.012 0.012 1.563 0.008 0.126
Residuals 168 1.304 0.008 NA 0.806 NA
Total 175 1.617 NA NA 1.000 NA
Fungi Habitat 1 0.552 0.552 5.004 0.026 0.001
Bedrock 1 0.644 0.644 5.843 0.030 0.001
Elevation 1 0.663 0.663 6.014 0.031 0.001
Habitat: bedrock 1 0.259 0.259 2.353 0.012 0.002
Habitat: elevation 1 0.137 0.137 1.244 0.006 0.199
Bedrock: elevation 1 0.232 0.232 2.102 0.011 0.010
Habitat: bedrock: elevation 1 0.182 0.182 1.654 0.009 0.041
Residuals 168 18.522 0.110 NA 0.874 NA
Total 175 21.191 NA NA 1.000 NA

The models also include the interaction effects on the microbial assemblages. Df, degrees of freedom; SS, sum of square; MS, mean square; FR, pseudo F-ratio; R2, partial variance explained by the factor, Pr(>F), P-value. Colons in factors indicate interactions. The effect of habitat type was significant regardless of the permutation strategy adopted, i.e., when samples were permuted within each Population, Transect, Massif or without strata (Table A3).