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. 2013 Mar 27;3:1553. doi: 10.1038/srep01553

Figure 2. Characterizing cellular uptake of siRNA from NanoRU.

Figure 2

(a) Scanning electron microscopy (SEM) image of NSCs on NanoRU. The NSCs (orange) and the NanoRU (blue) in the SEM image have been pseudocolored to enhance the contrast. Scale bar: 10 μm. Inset: Magnified view. Scale bar: 500 nm. (b) Quantitative comparison of the percentage of GFP knockdown in NSCs on NanoRU containing nanoparticles ranging in size from 100–700 nm. Analysis of variance (ANOVA) was used for evaluating the statistical significance of GFP knockdown (* = P < 0.05). (c) Phase and fluorescence images of NSCs on NanoRU with and without GFP siRNA. Scale bars: 20 μm. (d) Left column: Phase contrast and fluorescence image of NSCs grown on NanoRU coated with Silencer® Cy3-labeled control siRNA, and then reseeded in a 24-well plate. Right column: Phase image and fluorescence image of NSCs grown on a control substrate (bare glass surface without nanofeatures) coated with Silencer® Cy3-labeled control siRNA, and then re-seeded into a 24-well plate. Scale bars: 20 μm.