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. Author manuscript; available in PMC: 2013 Apr 26.
Published in final edited form as: Cell. 2012 Oct 11;151(3):533–546. doi: 10.1016/j.cell.2012.09.027

Figure 2. Loqs-PB Helps Dcr-1 Produce a Subset of miRNAs, Whereas Loqs-PD Helps Dcr-2 Produce Endo-siRNAs and Exo-siRNAs.

Figure 2

(A) Silencing of white mRNA by a long hairpin RNA trigger corresponding to white exon 3 (GMR-wIR) was measured by using eye pigment from 3- to 4-day-old female fly eyes. Data are mean ± SD for five biological replicates. Figure S2A presents data from male heads.

(B–D) Sequencing of small RNAs from female heads expressing the wIR RNAi trigger and from ovaries.

(B) Normalized number of reads (ppm) for esi-1.1, esi-1.2, and esi-2.1, white siRNAs, miRNA (red) and miRNA* (blue) from fly heads and ovaries. Only miRNAs and miRNA* strands whose abundance was > 100 ppm in at least one library were analyzed. Figure S2C presents additional head data.

(C) Abundance and 5′ position of wIR-derived siRNAs in heads. Antisense siRNAs are shown in red, sense in blue.

(D) Change in cis-NAT and transposon-derived endo-siRNA abundance in heads.

See also Figures S2, S3 and S4 and Tables S2, S3, and S4.