Figure 4.

PD-L1 on Batf3-dependent DCs is required for CTL cross-tolerance in the RLN. (A) Proportion of apoptotic OT-I cells transferred into wild-type (black) or Batf3^−/− (white) mice 18 hours after intravenous injection of 8 µg/g body weight OVA. (B) Experimental scheme for mixed bone marrow chimeras. Irradiated wild-type or PD-L1^−/− mice are reconstituted at a 1:1 ratio with bone marrow from Batf3^−/− mice and wild-type or PD-L1^−/− mice. (C) Proportion of apoptotic OT-I cells in the RLN and spleens of Batf3/wt (white) or Batf3/PD-L1 mixed chimeras (black) 18 hours after injecting 8 µg/g body weight OVA intravenously. Controls include OVA-injected (light gray) or noninjected wild-type mice (dark gray). (D) Same as in C except that PD-L1^−/− mice are used as bone marrow recipients. Results are representative of two independent experiments using four mice per group. wt, wild type; bm, bone marrow.