Fig. 8. FimX is required for cytotoxicity towards HeLa cells, but not for virulence in the murine model of acute pneumonia.
A. LDH release measured from infected HeLa cells. Triplicate wells of HeLa cells were infected with indicated bacterial strains at a moi of 10. Culture supernatants were collected at 2 and 4 hpi and assayed for LDH activity. Bars indicate mean ± SD of a representative experiment.
B. Bacterial recovery from infected mice in an acute pneumonia model. Eight- to 10-week-old female C57Bl/6 mice were infected with c. 5 × 105 cfu of PA103 (n = 8) or ΔfimX (n = 10). Mice were euthanized at 16 hpi and the number of bacteria present in lungs and liver were determined as described in Experimental procedures. Results are expressed as the ratio of cfu recovered per g tissue (output) to cfu present in the inoculum (input); each animal is represented by a data point, while the bar shows the geometric mean for each group. The Mann–Whitney test was used to calculate P-values (two-tailed) for each pairwise comparison indicated.