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Lipid profiles and distributions from direct tissue and MSI NIMS/MALDI experiments of C. borealis brain. (a) Zoomed-in lipid rich area (m/z 700 – 900) of MALDI direct tissue mass spectrum. (b) MALDI MS/MS spectra of PC 36:1 + K+ (m/z 826.58). (c) MALDI and NIMS distribution of PC 34:1 + K+ (m/z 798.56). (d) MALDI and NIMS distribution of PC 36:1 + K+ (m/z 826.58). (Scale bar = 1 mm)
