FIG. 3.
Quantification of β-cell proliferation by 7 days’ BrdU incorporation. A: BrdU in either 1 or 10% sucrose drinking water was supplied to the control TBR12F and the PTF1acreTBR12F mice for 1 week (1w) immediately after surgery. Representative BrdU (red) and insulin (INS) (green) staining is shown in sham-operated (top row), 50% PPX (middle row), and ligated part of PDL (bottom row) pancreas. HO, nuclear staining by Hoechst (blue). Scale bars = 50 μm. B: BrdU+ β-cell percentage was quantified in each experimental condition (1% sucrose: control sham solid blue, control PPX solid red, control PDL solid green; 10% sucrose, corresponding to the color in control from various treatments but diagonal lines instead of solid; insulin pellet treatment after PPX, cross-hatched red). Significance was considered when P < 0.05. P < 0.05: A and B, B and C, A and C, B and B’, D and E, D and F, E and F, A and D, B and E, a and b, b and c, b and b’, d and e, c and f, e and f, a and d, A and a, C and c, B and b, B’ and b’. No significance: a and c, d and f.