FIG. 6.

A: Bar graph shows mean ± SEM of Rac1 knockout efficiency in soleus and EDL muscle of muscle-specific inducible Rac1 KO mice and WT controls (n = 11–13). B: Representative Western blots showing the effect of doxycycline (1 g/L) treatment on Rac1 protein in WT vs. Floxed Rac1 KO mice (Rac1 fl/fl*Cre) (gastrocnemius muscle). C: Contraction-stimulated 2DG uptake in incubated isolated soleus and EDL muscles from WT and Rac1 KO mice (n = 11–13). D: Contraction-stimulated fold changes from basal in 2DG uptake in soleus and EDL from WT and Rac1 KO mice. E: Representative Western blots of p-AMPK^Thr172, p-ACC^Ser212, PAS, p-PAK^Thr423, p-TBC1D4^Thr642 (Soleus), p-TBC1D1^Ser237 (EDL), and actin, GLUT4, and Rac1 total proteins in response to contraction. F: Bar graph shows mean ± SEM of contraction-induced p-AMPK ^Thr172, p-ACC ^Ser212, PAS, p-PAK^Thr423/PAK1, p-TBC1D4^Thr542, and p-TBC1D1^Ser237 in soleus and EDL from Rac1 KO mice and WT controls. G: Bar graph shows mean ± SEM of total PAK1 and GLUT4 protein in soleus and EDL of WT and Rac1 KO mice. Statistical significances between basal and contraction are indicated by *P < 0.05; **P < 0.01; ***P < 0.001. Effect of genotype on contraction-stimulated 2-DG and p-PAK^Thr423 is indicated by #P < 0.05. Main effect of genotype is indicated by †P < 0.05; †††P < 0.001. Values represent mean ± SEM.