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. 2013 Jan 29;24(3):283–294. doi: 10.1089/hum.2012.162

FIG. 2.

FIG. 2.

SIV lentivirus transduction of mouse adipose tissue-derived stem/stromal cells (mADSCs). (A) The SIV lentiviral vectors used in this study expressed the gene of interest (GOI) under the control of the cytomegalovirus promoter (CMVprom). 3′ LTR (SIN), self-inactivating 3′ long terminal repeat. (B) The efficiency of transduction was determined by measuring the proportion of EGFP fluorescence-positive cells by flow cytometry. mADSCs were transduced with SIV-EGFP at MOIs of 0, 1, 5, 10, 30, and 60. Transduction efficiencies were analyzed by flow cytometry 96 hr after transduction. (C) Microscopy images of mADSCs transduced with SIV-human coagulation factor IX (hFIX) at MOIs of 0, 1, 5, 10, 30, and 60. Scale bar: 50 μm. The experiments were repeated three times with similar results.