FIG. 3.
SIV lentivirus-mediated human coagulation factor IX (hFIX) production from mouse adipose tissue-derived stem/stromal cells (mADSCs). mADSCs were transduced with the SIV-hFIX vector at MOIs of 1, 5, 10, 30, and 60. (A) The culture medium was collected 96 hr after transduction, and the amount of hFIX protein secreted over 24 hr was assessed by ELISA. mADSCs transduced with SIV-EGFP were used as the negative control. (B) The hFIX secretion profile from mADSCs was observed over 4 weeks. The culture medium from a 24-hr period was collected on days 4, 8, 19, and 28 after transduction. The amount of hFIX protein per 1×105 cells was measured by ELISA. (C) mADSC cell numbers were counted on days 4, 8, 19, and 28 after transduction. Cells treated at an MOI of 30 were unable to reach confluency until day 28. *p<0.05 between groups. †p<0.05 versus the other MOI groups; **p<0.05 between MOIs 1 and 10 on day 4 (n=3).