FIG. 4.

Clotting activity of human coagulation factor IX (hFIX) produced by mouse adipose tissue-derived stem/stromal cells (mADSCs). (A) mADSCs were transduced with the SIV-hFIX lentivirus vector at an MOI of 10. Seventy-two hours after vector transduction, the medium was changed to fresh medium containing menaquinone (vitamin K₂) at various concentrations (0, 0.1, 1, 5, 10, and 20 μg/ml). Twenty-four hours later, the culture supernatant was analyzed by a clotting assay. (B) The clotting activities of hFIX from mADSCs transduced at MOIs of 1, 5, 10, and 30 were analyzed 28 days after vector transduction. The medium was changed to fresh medium containing menaquinone (10 μg/ml) 24 hr before medium collection. *p<0.05 between groups (n=3).