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. 2013 Jan 29;24(3):283–294. doi: 10.1089/hum.2012.162

FIG. 6.

FIG. 6.

Cell sheets of lentivirus-transduced mouse adipose tissue-derived stem/stromal cells (mADSCs). (A) mADSCs were transduced with SIV-EGFP lentiviral vector and replated on a temperature-responsive culture surface (UpCell 35-mm dish) 96 hr after transduction. After reaching confluency on day 4 of culture, the culture temperature was lowered to 20°C to observe cell sheet harvesting. EGFP-transduced mADSCs started to detach from the edge as a cell sheet, shrank in size, detached, and floated in the medium after 20 min. Bottom right: High-magnification image of a floating EGFP-transduced cell sheet. The dashed circle represents the edge of the temperature-responsive culture dish (diameter, 35 mm). (B) SIV-hFIX-transduced mADSCs were replated on a temperature-responsive culture dish 96 hr after transduction. After 4 days of culture, the hFIX-transduced mADSCs were harvested as a cell sheet by lowering the culture temperature to 20°C and were reattached onto another culture dish. After 24 hr of culture, the medium was collected to measure hFIX protein levels by ELISA.