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Analysis of NIS mRNA expression in Huh7 tumors and nontarget organs by quantitative real-time polymerase chain reaction (qPCR). While only a low background level of NIS mRNA expression was detected in tumors injected with WT-MSCs, significant levels of NIS mRNA expression were induced in Huh7 tumors after three applications of RANTES-NIS-MSCs with or without NaClO4 pretreatment. In nontarget organs like liver, lung, kidney, or spleen, no NIS mRNA expression was detected.
