FIG. 2.

SDF-1 gene and protein expression in Ad-SDF-transduced chondrocytes cultured in 2D 24-well plates. Ten thousand cells were plated in each well and transduced with Ad-N at MOI 500 or Ad-SDF at MOI 100 and 500, respectively. (A) SDF-1 mRNA levels at MOI 100 and 500. At day 9, cells were lysed, and RNA was isolated and reverse transcribed for quantitative polymerase chain reaction analysis; (B) SDF-1 protein levels at MOI 100 and 500. The medium was collected every 3 days and used in ELISA for SDF-1 protein level determination. *p<0.05. MOI, multiplicities of infection.