Figure 2. Comparing infection rate using the simplified PEG/(NH₄)₂SO₄ two phase aqueous partitioning method and CsCl first generation method. Animal procedures and virus injection were preformed with prior approval of the University of Pittsburgh Institutional Animal Care and Use Committee (protocol number: 1103211). The anesthetized 10 weeks old CD1 mice (n = 3) were injected with 100 μl of AAV6-GFP (titer 2.3 × 10¹² GCP/ml) through the common bile duct. A clip was placed on the bile duct near the hilum of liver to clamp the hepatic duct, leading to specific perfusion of the pancreatic ducts. Pancreatic ducts infected with 10%PEG/13.2%(NH₄)₂SO₄ aqueous partitioning purified AAV6-GFP (A) or with first generation CsCl gradients ultrahigh speed centrifugation purified virus (C). (B and D) are merged counterstained with DBA for ducts (red), DAPI(blue) and GFP.