Table 1. Glucoamylolytic activity (nKat/DCW) of the engineered S. cerevisiae F2 and F6 strains once grown in YPD broth for 72 h.

	Soluble starch			Raw starch
	60°C	37°C	30°C	60°C	37°C	30°C
S. cerevisiae 27P	ND	ND	ND	ND	ND	ND
S. cerevisiae F2	3061 ± 215	833 ± 79	721 ± 56	1425 ± 90	396 ± 34	345 ± 30
S. cerevisiae F6	2380 ± 156	598 ± 48	515 ± 46	1169 ± 76	288 ± 23	251 ± 18

The assays were performed at 30 and 50°C in citrate-phosphate buffer at pH 4.5 with either 0.1% soluble starch or 2% raw starch. The values are the means of the results obtained from two experiments conducted in triplicate (± SD). ND: below detection limit.