Table 2. Ethanol production by S. cerevisiae strains engineered for the multiple integration of amylolytic genes.
S. cerevisiae strains | Sugara g/L |
Ethanol g/L |
Q (volumetric productivity) (g/L)/h |
Q max (g/L)/h |
q (specific productivity) g(g DCW)/h |
q max g(g DCW)/h |
Ethanol Yield |
Reference |
---|---|---|---|---|---|---|---|---|
Raw starch medium |
|
|
|
|
|
|
|
|
MT8–1SS |
110.00 |
26.0 after 84 h |
0.31 |
- |
- |
- |
0.45 (80%)b |
Yamada et al.28 |
NBRC1440SS |
110.00 |
28.0 after 84 h |
0.33 |
- |
- |
- |
0.52 (93%)b |
Yamada et al.28 |
MN8140SS |
110.00 |
39.0 after 84 h |
0.46 |
- |
- |
- |
0.44 (79%)b |
Yamada et al.28 |
F2 |
20.25 |
2.6 after 240 h |
0.01 |
0.016 (48 h) |
0.018 |
0.035 (48 h) |
0.42 (75%)b |
Favaro et al.20 |
F6 |
20.25 |
2.1 after 240 h |
0.01 |
0.010 (48 h) |
0.015 |
0.024 (48 h) |
0.41 (74%)b |
Favaro et al.20 |
Soluble starch medium |
|
|
|
|
|
|
|
|
SR93 |
55.00 |
14.3 after 140 h |
0.10 |
- |
- |
- |
0.48 (85%)b |
Nakamura et al.27 |
F2 |
20.25 |
5.4 after 48 h |
0.11 |
0.23 (18 h) |
0.040 |
0.12 (18 h) |
0.44 (79%)b |
Favaro et al.20 |
F6 |
20.25 |
4.8 after 48 h |
0.10 |
0.11 (18 h) |
0.037 |
0.08 (18 h) |
0.42 (76%)b |
Favaro et al.20 |
Glucose medium |
|
|
|
|
|
|
|
|
27P |
20.25 |
9.9 after 24 h |
0.41 |
0.70 (6 h) |
0.136 |
0.32 (6 h) |
0.49 (96%)c |
Favaro et al.20 |
F2 |
20.25 |
9.8 after 24 h |
0.41 |
0.64 (6 h) |
0.135 |
0.29 (6 h) |
0.49 (95%)c |
Favaro et al.20 |
F6 | 20.25 | 9.9 after 24 h | 0.41 | 0.66 (6 h) | 0.135 | 0.25 (6 h) | 0.49 (96%)c | Favaro et al.20 |
a Sugar amounts determined from the sum of starch and glucose in medium. Ethanol yield as g/g consumed sugar and % of theoretical maximum (0.56 g/g from starchb or 0.51 g/g from glucosec) indicated in brackets. S. cerevisiae F2 and F6 were studied in raw starch medium (20 g/L corn starch, 6.7 g/L yeast nitrogen base, 20 g/L peptone and 0.5 g/L glucose), soluble starch medium and glucose medium where the equivalent amount of raw starch was replaced with either soluble potato starch or glucose (Sigma).