FIG. 4.

AAV9-LARGE virus treatment ameliorated muscular dystrophy in the diaphragm of Large^myd mice. Diaphragm sections of 3–4-month-old mice were stained with hematoxylin and eosin (H&E). (A and C) are controls (low and high magnification). (B and D) Control treated with AAV9-LARGE (low and high magnification). No change in morphology was observed. (E and G) Large^myd mice (low and high magnification). Massive necrosis with inflammation (asterisk) and centrally located nuclei (arrows) were present. (F and H) Large^myd mice treated with AAV9-LARGE (low and high magnification). Note absence of necrosis and very few centrally located nuclei after AAV9-LARGE treatment. Scale bar in D, 50 μm. PBS, phosphate buffered saline.