FIG. 6.

AAV9-LARGE virus treatment reduced the number of regenerating fibers in skeletal muscle of Large^myd and POMGnT1 knockout mice. Diaphragm (A–I) and quadriceps muscle (J–X) sections from 3–4-month-old control and Large^myd and POMGnT1 knockout mice were immunofluorescence stained with antibodies against monoclonal rat antineural cell adhesion molecule (NCAM) (A,D, G, J, M, P, S, and V), developmental myosin heavy chain (MHCd) (B, E, H, K, N, Q, T, and W), 5-bromo-2’-deoxyuridine (BrdU) (C, F, I, L, O, R, U, and X). (A–C and J–L) Control diaphragm and quadriceps muscle sections are stained with antibodies against NCAM, MHCd, and BrdU respectively. (D–F and M–O) Large^myd diaphragm and quadriceps muscle sections stained with anti-NCAM, MHCd, and BrdU respectively, showing presence of NCAM- and MHCd-positive myofibers and BrdU-labeled nuclei. (G–I and P–R) Diaphragm and quadriceps muscle sections of AAV9-LARGE-treated Large^myd mice are stained with antibodies against NCAM and MHCd and BrdU respectively. Note absence of NCAM- and MHCd-positive fibers and BrdU-labeled nuclei in AAV9-LARGE-treated Large^myd samples. (S–U) POMGnT1 knockout quadriceps muscle showing presence of NCAM- and MHCd-positive fibers but absence of BrdU incorporation. (V–X) AAV9-LARGE virus treatment reduced NCAM- and MHCd-positive fibers in POMGnT1 knockout mice. A-L, AAV9-LARGE. Scale bar in X, 50 μm.