FIG. 5.

Effect of F108 selective seeding of human MSCs in collagen microchannels. MSCs prestained with Vybrant were seeded onto F108-treated collagen linear channels overnight. Fluorescent images of cells in linear channels of widths 25 μm (A), 50 μm (B), 100 μm (C), 500 μm (D), and 1000 μm (E) show selective seeding compared to untreated channel surfaces (F). Insets in (D) and (E) show magnified portions of gaps (50 μm) between the channels devoid of cells. Scale bars: 50 μm (A, B, F), 100 μm (C), 200 μm (D), 400 μm (E). Color images available online at www.liebertpub.com/tea