Table 1.

Cell Alignment Angle During Contact Guidance

Channel width (μm)	25	50	100	500	1000
PDMS	3.7±1.4a	12.7±2.4a	4.6±1.0a	29.2±2.8	32.2±4.2
Collagen	4.5±0.8a	4.1±0.6a	8.6±1.0a	41.8±2.5	42.3±3.0

Angle between the linear channel dimension and cell major axis through the cell nucleus was quantified and shown as mean±SEM for channels of various widths. For smaller channels (25, 50, 100 μm), six channels chosen from three different experiments were used for quantitation. For larger channels (500, 1000 μm), three channels from three different experiments were used. Cells from three donors were used in the experiments. Corresponding immunofluorescence data are shown in Figure 6A–E.

^aStatistically significant (p<0.05) compared to 500- and 1000-μm-wide channels.

PDMS, polydimethylsiloxane.