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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Breast Cancer Res Treat. 2011 Sep 14;133(2):437–445. doi: 10.1007/s10549-011-1763-0

Fig. 2.

Fig. 2

Flow cytometry analysis of caspase activation in CD44+/CD24−/ALDH+ subpopulation of 2LMP and SUM159 cells after treatment with TRA-8. 2LMP (a) and SUM159 (b) cells were sorted for the ALDH+ subpopulation and then treated with TRA-8 or control IgG for 2 h. Cells were then fixed and stained for CD44 (APC), CD24 (PE), and activated caspases 3 or 8 (secondary Alexa 405). 2LMP and SUM159 BrCSC enriched cells had caspase 3 and caspase 8 activation (green line) after incubation with TRA-8 compared with IgG control (red line)