Fig. 3.

Secondary tumorsphere formation inhibition by TRA-8. 2LMP cells (a) and SUM159 (b) cells were sorted using flow cytometry for ALDH⁺ cells and allowed to form primary tumorspheres for 3 days. After tumorspheres were mechanically dissociated, single cells (2,000 cells/well) were plated in low attachment plates and treated with IgG, TRAIL, 2E12 (anti-DR4), TRA-8, adriamycin or taxol. After 48 h, tumorspheres ranging from 40 to 120 μm in size were visually counted using a reticle eye piece. Mean tumorsphere inhibition was calculated relative to untreated controls (blue bars) (filled square) represent median values. Error bars represent SD of the samples run in quadruplicate