(A) The expression of the human neuron-associated miRNA group 1 was expressed at low levels in pluripotent hESCs, slightly up-regulated in hESC-I hNuPs, and displayed drastic increases of expression to high levels in further differentiated hESC-I hNus. (B) The expression of the human neuron-associated miRNA group 2 was down-regulated to low levels of expression in hESC-I hNuPs, and then significantly up-regulated to high levels of expression in further differentiated hESC-I hNus. (C) The expression of the human neuron-associated miRNA group 3, including the let-7 miRNA family, was expressed at low levels in pluripotent hESCs, and significantly up-regulated in the progression of hESC neuronal lineage specific differentiation to high levels of expression in hESC-I hNus. (D) The expression of the human neuron-associated miRNA group 4 was up-regulated in hESC-I hNus, albeit to less extents. (E) Log2 ratios of up-regulation of human neuron-associated miRNA group 1–3. The levels of miRNA expression up-regulation in (A)–(D): * 5-fold ---10-fold, ** 10-fold ---100-fold, and *** 100-fold --- 500-fold. All microarray data are mean values from at least two biological replicate sets, standard derivations were not shown.