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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Mol Med Ther. 2012 Dec 10;1(2):105. doi: 10.4172/2324-8769.1000105

Figure 7. The neuroectoderm-derived human neuronal progenitors specified from hESCs are highly neurogenic following transplantation and in 3D culture.

Figure 7

(A) hESC-I hNuPs were injected into the cerebral ventricles of newborn mice. Histological analysis of transplanted mice showed well-dispersed and well-integrated human neurons at a high prevalence, indicated by anti-human mitochondrial antibody (hMit) (red) and their immunoreactivity to Map-2 (green), including Nurr1-positve (green) and TH-positive (green) DA neurons, within neurogenic regions of the brain. DAPI nuclear marker (blue) stains all cells in the field. (B) Under neuronal subtype specification conditions in 3D culture, these hESC-derived neuronal cells (expressing Map-2 and co-expressing βIII-tubulin) further proceeded to express subtype neuronal markers associated with ventrally-located neuronal populations, such as TH (DA neurons) and Hb9/Lim3/Isl1 (motor neurons) (shown in 3D matrix). All cells are indicated by DAPI staining of their nuclei (blue).