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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: J Regen Med Tissue Eng. 2012 Sep 23;1(1):3. doi: 10.7243/2050-1218-1-3

Figure 6. The neuroectoderm-derived hESC neuronal lineage-specific derivative is highly neurogenic in vitro and in vivo.

Figure 6

(A). Under neuronal differentiation conditions, hESC-I hNuPs yielded exclusively neurons that expressed neuronal marker β-III-tubulin and co-expressed Map-2 with a drastic increase in efficiency when compared to similarly cultured cells derived from un treated embryoid bodies (EBs) as the control. No cell expressed glial lineage marker GFAP and MBP. Accordingly, a large proportion of these hESC-derived neuronal cells began to express tyrosine hydroxylase (TH).

(B). hESC-I hNuPs were injected into the cerebral ventricles of newborn mice. Histological analysis of transplanted mice showed well-dispersed and well-integrated human neurons at a high prevalence, indicated by anti-human mitochondrial antibody (hMit) and their immunoreactivity to Map-2, including Nurr1-positve and TH-positive DA neurons, within neurogenic regions of the brain. DAPI nuclear marker stains all cells in the field.