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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
CART increases ATP synthesis in purified neuronal mitochondria. Mitochondria were extracted from cultured primary cortex neuronal cells and treated directly with CART peptide or vehicle (control). ATP levels were measured using a luciferase-based bioluminescence detection assay.