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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: J Chromatogr A. 2008 Sep 12;1210(1):55–59. doi: 10.1016/j.chroma.2008.09.022

Fig. 2.

Fig. 2

T1AM was detected from serum with LC-MS/MS experiments monitoring for the transition from m/z 356 precursor to either m/z 339 or 212 product ion (A). Monitoring for the transition to m/z 212 product ion resulted in extracted ion chromatograms demonstrating the detection of 500 fmol on-column T1AM injection from 0.2 mL of human and 2.5 mL rat serum (LLOQ calibrator). Detection of endogenous T1AM from 0.2 mL of human, mouse and rat serum was also observed (B). The retention time of T1AM (designated by *) was 5.5 min.