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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: J Chromatogr A. 2008 Sep 12;1210(1):55–59. doi: 10.1016/j.chroma.2008.09.022

Fig. 3.

Fig. 3

Calibration curves for quantifying T1AM were generated by a linear regression of the peak area ratios (T1AM/T1AM-d4) determined for rat serum calibrants versus specified T1AM concentration (standard deviation indicated, A). T1AM was quantified from rat serum (n=3) after IV injection with 20 mg/kg T1AM. A semi-logarithmic plot is shown for serum concentration versus time (standard deviation indicated, B). A linear regression for the elimination phase for T1AM resulted in an equation y = −0.0146x + 0.276 with a correlation coefficient (r2) = 0.999. From the slope K was determined as 0.0146 min−1, allowing calculation of a biological half-life (t1/2) value for T1AM of 48 min.