T1AM metabolites in mouse serum. A) Concentrations of T1AM, T1AM-glucuronide, S-T1AM, Ac-T1AM, TA1 and TA0 between 10 minutes and 6 hours after a single IP injection of T1AM. Inset shows data from 0 to 2 hours; n = 3–4 per point, except for the following: T1AM and T1AM-glucuronide, 6 hours, n = 2; Ac-T1AM, 3 hours, n = 1; TA0, 10 and 45 minutes, n = 2; TA0, 20 minutes and 3 hours, n = 1. B) Representative chromatographic traces of T0AM in serum at 20 minutes post-T1AM injection in the absence (left) or presence (right) of β-glucuronidase.