Figure 3. Podosome turnover is not affected by SWAP-70 deficiency in DCs.

(A) FRAP analysis. Actin-GFP transduced Swap-70^+/+ and Swap-70^–/– DCs were seeded on poly-L-lysine coated glass coverslips and discrete areas (white circles) were photobleached. Representative selected pictures are shown with times in seconds. (B) Quantification of fluorescence recovery half-time (t_1/2) in seconds from experiments in (A). Mean values of 7 individual photobleached cells per genotype of 2 independent experiments with error bars indicating ±SD are shown. N.S. denotes not significant.