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. 2013 Mar 27;8(3):e60642. doi: 10.1371/journal.pone.0060642

Figure 4. Podosome-mediated degradation of gelatin is not affected by SWAP-70 deficiency in DCs.

Figure 4

(A-B) Confocal immunofluorescence microscopy analysis of gelatin degradation by Swap-70+/+ and Swap-70–/– DCs. DCs were seeded on Oregon Green-labeled gelatin (green) and incubated for 4 h. Cells were stained with rhodamine phalloidin for F-actin (red) and DAPI for the nucleus (blue). Representative detailed (A) and overview (B) pictures are shown. (C) Quantification of the area (in µm2) of Oregon Green-labeled gelatin degraded by DCs. Mean values of 3 independent experiments with error bars indicating +SD are shown (300-500 cells were analyzed per genotype and experiment). N.S. denotes not significant.