Figure 2. R cell fate, lamina neuron development, and glia cell development are normal.

Eye discs stained with anti-Elav (A–C), anti-Prospero (D–F), and anti-Senseless (G–I) showed normal staining pattern for all R cells, R7 and R8, respectively. Brains stained with anti-Dachshund (J–L) showed normal differentiation of lamina neurons. (M–O) Eye-brain complexes double stained with 24B10 (green) for R cell axons and anti-Repo (red) for glial cells. Lamina plexus (green, between two check markers) was present between two layers of glial cells (red), epithelial glia and marginal glia. The number of glial cells in R1-6 target region in wgn and moe mutants was similar to wild-type. This indicated that the differentiation and migration of glial cells were normal in wgn and moe mutants. The array of glia around lamina plexus was mildly disorganized in both mutants. This was likely caused by abnormal projection of R cell axons. Arrows indicate glial cells. Abbreviations: lp, lamina plexus; eg, epithelial glia; mg, marginal glia. Scale bars: A–C, D–F, and G–I, 10 µm; J–L and M–O, 20 µm.