Figure 5. Wengen and Moesin interact physically and genetically.

(A) Alignment of MPD domain in Wgn and human Fas, with clusters of basic amino acids underlined. (B) Lysates prepared from fly heads (w¹¹¹⁸; GMR-Gal4/UAS-moe-Myc) were used in the pull down assays with immobilized GST, GST-ΔECD-ΔMPD, or GST-ΔECD. Pull downs were analyzed by anti-Myc immunoblot (upper panel). The amount of GST, GST-ΔECD-ΔMPD and GST-ΔECD proteins used for pullouts were shown by Ponceau red staining (lower panel). (C–G) Eye-brain complexes were stained by 24B10. R cell axon projections were normal in wild-type (C) and moe^G0323/+ (F) heterozygous animals. (D) wgn^e00637 mutant. Lamina plexus was disrupted. It appears uneven and has a gap indicated by arrow head. (G) Removing one copy of moe^G0323 enhanced the wgn^e00637 phenotype with large gaps apparent in the lamina plexus (arrow heads). (H) Quantifications of genetic interactions. By removing one copy of moe in wgn^e00637 mutant background, the penetrance of wgn^e00637 increased from 70% to 100%. Scale bar, 10 µm.